Development of production methods for recombinant protein

Applicable for

  • recombinant proteins produced in E.coli (cytoplasmic or periplasmic expression) and P. pastoris (secretion).

Services proposed

  • Construction of protein expression systems (E. coli or P. pastoris strains):
    • vectors design including selection between different strains, vectors, selection markers, promoters, secretion signals, coding sequences, modes of codon optimization.
    • transformation, clones screening and selection of the best producing clones.
  • Fermentation:
    • screening of optimal process conditions
  • Harvest and extraction:
    • optimization of the harvest conditions for cells or media recovery
    • development of the extraction conditions: high pressure treatment, osmotic shock, chemical lysis
    • preparation, solubilization and refolding of inclusion bodies
  • Chromatography:
    • high throughput screening of resins and chromatographic conditions
    • validation of resins and fine-tuning of process conditions using small-scale columns
  • Ultrafiltration and microfiltration:
    • membranes and process conditions screening
  • Industrial transfer:
    • scaling-up studies (chemical engineering)
    • multi-parameters process monitoring


  • PetriResearch Cell Banks (50 vials) with certificate of analysis
  • Purified material for in vitro trials (activity testing, formulation studies…).
  • Development report including:
    • method of production
    • operating parameters at the different scales of production
  • A process transferable to the Xpress Biologics GLP platform.
  • A process “ready-to-transfer” to GMP manufacturing.